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to a prec?.omlnant extrnt. clegradstfoa products o f racbn. h e is bpresaed by the hi@ counting rates obtained e ~ a ~whsm the orig hal rabn concentration l e as low am I t is iu norml air, Eased upon this obsem$loa, one poesibla o f expired ait frcn the l ~ d5vldw lto be maswede o f very fins dust in this air and keep i t in suspension over a period long a n o w for the growth of a csnsfdersble carimnf; of Fa% ia a thin layer snd ffia30ur9 Its alp la activityo 3 ) Collect thio dust pIBJ&horl a r k cn thks mtkod o f &py;soashhac besn dropped for the presoat because o f the e.qcrirnsu9;al difficultieo encountsrcQ and hceaaoe aao*&er method Bce?iis to of fe ~a batter ap-mach t o the s ~ lutlo nof this prcblem Experimental Teats of thin Metho& ha mentioned in our progteas report for B vember, i t has bean found that 'relatively atrang ndon twnpleu (I.eo cfiarcoal at room temperature, over the charcoal, S5$ of the orig;& dovelopmeat as paasi0le in this direction, I lo"g curfew per liter) rmy be 9 9 absorbed by passing over 5 grams o f activated matbbd seeme paamislag enough that w3 propose t~ camp o a t a8 intensive a 1 rabn gas mziple CEKI b e recovoredo This
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20 1 Intranuscular Injectinns o f Il3fl ’3it;hZol Coqousds as bla ted to the MetaboliEm of Poatwi carried out under his direcZion during t h e past few aonths) The loarksd success obtalned in tue use of certain NDB d i t b i o l compounds, elements such a s arsenic m d cadmiwn suggested that exporimerata be p8rfOrRed to t e s t whe3her any of the available ED2 compounds would increase the rate a t which po’lonbw was excreted. The objective of these experimects was to aid in aevisfng therapextic me&sliree t o be uesd in case ~f acciiiejztal .?agestion o f poloniw. Prd iainary snawers wale thsrsfore sought to the specific question8 hiale rats were the experimental animals us& the order in which the experiments were dona, per kilogram body weight, After injection of the rat, an equal ammt of the injection solution was teken ur, in the syringa 2nd f.ischtged i n t o a bottle A n slfnuot o f this solution vas anztyzad to es tsb ll ch ths theamtical. dose, Since stunies of polonium metatolism conducC,sZ Ln this laboratory hve Each rat was weigimd and a adminis tm tion, k,t h intravenous and subcutzreous inject do as vers used in carrying o u t the experiments presented here. 1;ztrsvenms in4cctions were made int ~the tail vein, and subcutaneous injectioos were m-de ia the right forelegh+,ramuscular Injections o f tho mil compo~xlm6er stxly were begun eithsr directly after tne polanium nad been abin ict ered ar a: chosen times saecified peanut o i l D this vehicle wes used, othemlse the sc;l~altw a 8 L S 5 3ercent sodium without prorluctng seriocs .toxic effects in the rat, 2 to 3 hour intervals and t o continue the tszattsent, for three rfzys, might bta are girea i n %ble ffor all of the .eqerimentsl rstsXI the 3DR c~mpoari?w s soluble i n ‘ h e dosage o f IUX? corqouad was mde as large as possible The dosage schedule varied . for the cont xl rats csed for conparison in this report bre tiken from the
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The rats were kep% an sepsmts glass mta bol isn cages and 24 hour collections were made of fecea cnd of urine, and seleceed tissues including $he InJeciinn s i t e were disrzcted out for A de^eiled discussion of tihe constnaction of tho cages xi well a s the tcchniqUe Ths remainder of t h e ra$ carmiss WEIS digastecl mad an aqalysis umde. In fact, $he dose is, in the fol lo xing data, d.t - j ~ ~ ~t o be undoratood as equal to a slannstioc nsRe i a ^ohis wayo to confom with other r q o r t s published bx the grm? sti~c:Qingnetabolisn of ;yoloniun in this laboratory acd I s jusiificd in p,sr*t by $ha circusstance t b t the theoretical dose arrivcd F.t by a n ~ lysi sof the ''durqy bj ect lon " does not take in%o a c c o u t such 2 ~ m r 5as incomploto nix-ir1g of tfic indjecticon sofution and ' plating 01.12 of p loni urn %SA the s y ~ inga dose as listed in Tab18 I vi11 shov t h t the dffTeaencea are usually sn;sll~
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elimination of polonium in feces for the tres team6 untreated ankle is
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The Fecal Excretion of In tre venous ly Administered Polonium -A fter Intrsmuscular I rij ect ions of BEL
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' h e latter interpretathon m~ybe discounted t o some extent for the NDR 399 rat by refsrence to the urfaary excretion table which shows a rete 4 times that o f the controls on the tenth .?ay, and indicates cn adcpato conccntretion of urinary excretion process i s concernoc'. Ihe table far urinrry excretion of polonium reveals another interesting and ? ossi bly significant finding; io e. rate b f urinary excretion of poboniuin on the first +.y i s from 2 to 5 tines shows thrt this is a l s ~the MSC for the rat,$ vhich reccivsd th,?polonium as 811 Intravenous in3 ec tion, Table 6 in terms of px cent of absarbsd rioac per gia ~w ~ s tweighte !!!he tissue data for the subcutaneous onba1.s i o presented in t0 that found for the intravenoas mimalEn 3 % r q - yJei"hpc Ple noted that 6 s h the intravenous d a b fhe percout absorb& dose 3 2 ~gzan was higher for muscle tissue of the ?lAL a n f i d s than for muscle tissue of tho othe? tzisated and is t b t the liver value for the EIDR 399 mima1 w a s low ia con?i.aa: to t h e very high mlue found in til3 EfDR 399 rats ifhen the ~01~miufichad been given as an in travenwis inject iono The questiocs puszd et the bsgiming of this in7cstigaf4oo have been answered in a 1irnid;ed fashionn The data on rats show that the W t a l eli m hati cn of polonium fo,r the first, 10 days after injection C m be sign ifk at lg increased by treatsent with certain HC? cor;lpous,C;so appeers t o devend on the &de of a&dtais%ration of pbo nim. effective of the various conpounds tested if the polonlun Las b2en bj ec ted intraveno-ssly; whereas Ts3R 399 or HDE 317 ara mre effective If ths polonilem The choice o f coiapogad
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