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which the a b - dioxide db d ampler Ir uulyzed for carboa lsotape . POadinQ to 0.35 g C-13. Breath sampler were collected in 50 ml ctrcuatcd b a r md rho carbon dioxide was Isolated cryqcnically as follows: Flrrt, Iht enate sample wai chilled with llqufd n lt qc n and a11 non-condensiblor were removed by pumflng. Tho mass-28 peak of the bulb was obscrved directly by the mass spcctromotcr to verify complcta removal of the643 gasor. dioxfde nas released, typtcrlly rcrching I9 to 12 mm pressure. A portlon of Ws pas u-3s malyzcd for the marr-44, 45, and 46 peaks on a Model 21-62OA mass spectrometer, manufachlred by Consolid&tcd Elcctrodynornics CorporntIm, PlloQaa, Cdlf. Total time for processlng and analyzing 3 sample v a r about of Ihe mass45 pek to tho total C02 peaks. Rcfercnca smplrr taken immcldlatcly boforo and well after tho test gave idcnticd values, I.lbt$, Renultr are dtrplaycd Ln Ffgs. 1 and 2 in t e r m s of tho percent contribution Alihough Lha pupow of h i 8 oxpcrirnent*doornot prorume to Include fnter- * pmt8Uorr of UIO pbysiologlcd processes nblch are Involved In the fato of COS . La mltzar wster, rsvfi detalle seem obvlwr. The Inl#al Mgh due bropplng
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. rapldly Is due to tho cndcbed CO, cnterSag the l&r directly during the the of drldilng tha sample. This it3 a Nghly carbonated rolutioa and qJto b&bly. The normal ven#lation of tho lungs diluter a18 lnitlol dose rapidly * .- In rrunmary, Lt bas been s h m that the technfqw of arulyzing the Isstoplo labdad compounds may be made rfmply, rapidly, occuratclq, and scnsftivcly. It should bc emphnsftcd that the mass spcctromctcr used for t h i s cxpertment Is= specially destgned. To the contrary, it is normally used for chcmfcal procorr control and is subjected to wfdclp \*aryinpIrotopfc composttionv of msny dfffcrcnt. gases. There is every reason to bclicve ih3t improvements of sensltivlty to milions from normal abundance are posstble l o the extent of n factor of 10 "biotop.tu1n0tsc'' so that curves roch as those of Fig. 2 are susceptible io accurato quYltfncatfon and analysis. This is the first demonstratioa of ruch ladebtod to Joe 0. Montoya for mccb+cd md pnalytical
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