Annual Report, Biomedical Research Group, Health Division La-1690 (Redacted)

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LOS ALAMOS SCIENnFIC LABORATORY UNIVERSITY OF CALIFORNU ANNUAL REPORT, BIOMEDICAL RESEARCH GROUP I HEALTH AND SAFETY

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2.1.3 Rog r sr of Specific Rojeets ........... 13 2.2.1 General Commnts on Section Activities ....... 23 2.3 Radio pt koli y Sostion .................. LO 2.3.1 Senera1 Commnts o n Section Activitirs ....... LO 2.3.3 Rogrerr of Specific Rejects........... 112 . L biophysics Section .................... 45 24.1 Canorel SoPLaonts on Section Activities ....... 45 2.1.2 Articles a d I)oc?rwnts 3ig irn ting in the Section . 12 2.2.2 Articlca and Docunentr Crigirmting f n the Saction . a 2.4.3 Progress of Specific Project8 ........... 17 2.5.2 Articles and Docmants '3r:girnting in tha Section . 52 2.5.3 hogre ~ ro f Sprcffic Projects ........... 53 3 . Roposcld Program for 1954 ................... 62 3.5 Orgrnic Cherirtry Section ................. 70 3.3 Wi o path oh gy Saction .................. 66

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out jointly with Wolph Fiold School of Arirtion lCd lc in ard c o ~ irtod Thore rerults vera reported i n During 1953 the studies verb rep..- using refizid t.chnigwr, ami ObWrVStioM of fncapacitrtion d p.rform3ce dofact from mlslva, rapid d o n 8 of grrr radiation. No spocffic dotal18 of rrrultr u'o given here. They w i l l k reported in 4 jolnt cl.8rifi.d roport from Group H-4, IASL,and th Schod of Aviation Mi cine. tiro radhtion h r u d from extornrl grrrar radiation aril fro8 inhaled firrion product# resuLtia(l fro0 wrly prugm through th. mushroom b e d of an --In rerponrcr t o tho mad for eXt.nriro furrdrolrnt.1 kncruledgr grrm into tho ckamistry and chractarirticr of liquid aolution rcintiIhtor8

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powdr . EJesfon op oct n of 3 g/l toluaru rclutionr of thoro solutes have b o a run with radium excitation, Eaorgr tran8far ktuoon rolutor h8 absorption rpectra in cy clo henn solution a& photon moan f’rao pth data have b s n obbirud. Solvent8 hvo been rtudird a8 to their light abwrption mi ch m lal propertior of poculiu intaroat in rc fn til h tion vork. of tortilq have k e n applied to liquid rolution rcintKlatorr pioldlng informtion diractly applicable to counting problsu. logic il inter art, mtural CU counting to the fah i cation of largo dwolap .ad apply a numbor of -l&n bio logi d tart ryrt-8 to the dery r tar tho offmctivrnrr of vu i our radiation8 or in tno rt to tho Lor Ahmr laboratory are bow det dr rd roh t ivo to tho offoct produmd b.r

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either oporrkd at I conrtant povrr levo1 otor a ralativr4 long pori& o f tim or dor up u a it i al for a fmr oicrowconir. given off is prodoPrinntlp fisrion nutronr. Som fission my8 .PO ilao given off but this ir b.1iov.d to conrtituta loss than 10 prr cmt

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In Augurt of 1953, the new Bio rn rdi ul Remuch kborrtorp uas turd ovor t o tha Unirarrity of California .nd occupied Umodl~toly. The building ual officially de8igmt.d as tha H u l t h Rawuch kboratory (St). The structure con trim laboratorirs, adminlrtrativa offitor, aid library, mmlnr room, miml q w tars, uork rhopr, photopaphfc drrboorr, X-ray axporure room, cobalt rource mxporurr room, a spacial counting room, atock and

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in tho bo Alamor horplul. Tha RIV X-ray unit Y.s not ymt iatrllad in fa ell tt loo wre provided in HRL for tho inrtrllrtion ofa cocuramnt of tb rource. installation of a luge cobalt aourco will kon of tbr rjor oquipaat At the r d of 1953 uranguontr hd not b o a mdo for pro-

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Tha 8-r uploy.rnt progru us# curtailed during 1953 hacause of the ant fci pt.d move t o MtL during tho 8 r " f wnthr. A much grutar mmor rplqmoat progrrn fr

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Tho follarlng puhllertion, or documntr woro frrual by tho vuiow 8-f ylbori of tha Biochalrtry Sactian d r ~ l n q1953. oa tho Syntharim of Cholo8t.rol i n Dog Tir8w & 'litlp. J. Bioi. Chu., Factor. Controlling Cholrskrol Synthorir i n thlr Body, f . UJW of Calcium Ethylondi.rilwt0trucet.t. in Cau of

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J, Rolo of Trace Elrwntr in An irl Car.A Tabulation Ikndbook of Biological Dstr to bo publirhd tho ICltioMl Ron uch k. Tho ktaboliam of C u - & b ldL thy lond Luin to tru a tic A Contimow Titration bthod for Cholinrtrrur Detormi-1.

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f a d i n g T-kkld cbolorkrol ami follouing tho turaovor rata of blood chorat.$ of hepatic ryntbrrlr dotorrirwd. fncrurrr turnovrr t f u ami docruwr rat. of 8yntharir but t& chng, i s not marly w rtrlkinq u in tu0 pt lon tr with @- r tud ld at tha kid it iom l rtudior on r8tr u o hiag curid out to dotaraim if it is tb iacrwm In liror mrt.rif1.d or livor fro. cholortorol frrctionr tht la rorponriblo for tha r h r p docmaw in rata of ryntherir ob0orv.d .fur incrwn in dio tu y cholortnol. Thir motbod fr bin#appliod to tho 1dentUfcrtlon of h tty acids proroat A moria# of nota h-M u3d a rorior of ur dio vu cul u th rprclfic actiriti of plam fro., oatarLfiod urd rod coll cholort.ro1 fallouod. Conrirt.at r orul tr for tho controls wro obtrilvd but additiolul

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proc.durO Y.S reputed using t h e kUoEDTA &nd the rorults of the tuo a*crotion ud blood clearance nu8ur.d kfore and after skin appllcrtion o f of Ca EI fiA in r wrkr soluble ointwnt. Thaw strdirr a n i n progrerr and so far indicate tmt EUTA did not eury mwrlrrablr amount6 of kuo ~ croa rthe akin brrfer. The question of the significance of buo-kUoactivity in the Urim of worker8 expod of th8 corr ol ~ tio nof urinuy rxcretion vith body burden. carrying out thr 8tudy In humnr, a study %madona vith ratr, zmmdy to develop tachniquor ard to check tho rorults of previour lnvortiptfons. gave 8010. additiornl informtion. Tho rerultr vorified provtour findings and The body milks the k froo an in j oct d b-& mhturr. The b f8 -en up principally i n the lfvrr, aomo by tho othor r o f t tissttas a d vary litt lr in the akoloton. Tho 8. gems priasipaUy t o tha skelatcn, TIS body wbc ti vel yu c ret or thr 8. i n both thr uri ~and fOC.8. than two pet cent of La was ox ao td in four by^. contrnt i n tirruoa and oxcrrta without ramortine to actual chomiml 0 8 p - counting wing a N.1 crystal. Tho proceduro imolvor thr urn of scintillation

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The rtufy in humna hrken ret up and Is i n procerr. of cold and hbld ironiarld MI izdectod prior t o placing tb. a w n in tho nuubolic caps. Cubon dioxida, feco ~ ,win8 and tirrlur vorr anlyred ciant aninul8 and 4 control# vera anmod a t om hour, 6 deficirnt ud doficient ardaBh i n th8 tirnu bmlyrir rrcept th turin u y rrcrrtion in tho deficient 8 ~ h m l rtr delayed. I s 0xcret.d i n tha urine inrk hour8 if the a- hra nod axerrtory raw. Elsentially all the drug fr ex c re td in twenty-four hours. CaI8iVa ccncontration of the drug vas fowd i n tha lungs 18 h r k o a reported i n tha muse. Ckorvtography of urimry a d tirrw mtabollter i a Approx ht aly -90 par cent of the drug Sucom 180 in log nu e lar WIT. wcriiicd fit. ~ nute raftar injection with C'-nitrogoa nurtard. In trau to rid inJoctionr vera uda into th

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r&minal ROT-, and intrsvenour injection8 into t h e jugulrr rima. t it it y of tho drug uhon given intra - ut.riJly. 8 lower rpc if ic activity with intrr-utarirl lajoction. routa tln LD50 of nitroeen m t u d given intra-utorlallgr VII 1.25 mg/kgl Rata boaring tho Walker tumr vat. 4 b c t . d intrautari8zd wctioar turd 0t.r t o hirtopthologp for Picrorcopic w nrt ion to find any ruianwr in tirrur dwg. whoa th drug -8 ghoa by difforont Autoprier urre don, A mtrbolic rtudy of dfoim tkld in tho 1ad ? porittoar y.8 mdo, Rolininuy work shovod th ttir rw r ud urine cou wbon dried inair without app liut ion of hut.

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nicm have ken subjected t o varying dous of X rays ~ YOE 50 r to 525 t, and the choll ~ star88 0valuer i n the red cella obt.ined on t h 8 fourth dry post-radiation. the controls) b v oken found atall do88 10VOlS. p.rOAt correlation ktweon do80 a d Mgnitudo o f effect. variations are 80 groat tbta very lug. nukr of a niu lr uould k requird to detect aut hut ical relatiomhip if rueh 8xirtr. profitable m y to ext8nd thlr uork Is probnbly to UII h r p r an-, BO tht chng.r can bo dstectod i n individuals. So far thero is no 8 p Idi vid tul T h fourth day hign valuer ufth subsequent rapid f.11 8re difficult to interpret 8t tho prosent s U @ , but hvo several interesting marrou, a relearn of young cells in numbrr too small to give a reticuloreturn to tha mrml levol. Uht bp pon r to the88 young calla i n the irradiated alce tr not known a t present. Another quaustion to k anmerod i r uhether the obwnod value, refarred t o the A O ~18001 for the inspread of &t. is found thnat other thar, ha8 progmrtic rrlw. The invortig8tioa rhould alw bo u t a r h d to dam# &merthan 50 rs rfnce th, th. doma recoivod by tho bon mrrw io an ir dlr idr vl on myom oeuslon. Th8 riw prrruarbly reflects 8 tr8nsisnt ruc tt on of the bone The sudden drop i s unprocadentd in ~ n yc old it tow so far o b lphoral red cell ptaturr vith do-8 of 300 f 8nd le88 fr in it wu of

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Thr act it it i or of the Rdfobiolom bction duriry 1953 -0 a. Obunntionr of long term affect8 of ringh ard et i plo d o n o of 1 and &rrr rayr ami nutrons. b. Studier of tha .cub affoct. of milive, rapid d o w , of d. Studieo of tho machnism of biological Bction of w i o u r

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h. Put ici pti on in group projects on of fot tr of ~ iiiro John Furchar took yar blur e of 8bwlrea to collplete trriabg for his Ph.D. dogroe. bjot Fayno Hurir, upon canplation of hL8 tow o f duty a t John Starm left for tu0 yurt8 retrice *, Tar bora workod t.lp on r ilyin the Group an 4 0- uplopo. Tho follarinq roporta, nitkn in vhok or in put rrkrr of th. Rdiobiolom Soation, vera isrtud or In rawcript bY th old of b. Studi.r on tho Mhnir of btba of Thd Poutronr in Roduclng B fol o ql a Effoctr, I, Lack of Eifwt of ttVpa0l.i. rad Clutrthiom

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rttu lungr without the 8id of tb on co to q 8Ird than locatad in tha lung8 by

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pupa of mico in ring10 rcub exposur08 adin fraction, of from four tm additivity of multipla neutron exposurer with the additivity o f multiple tb chronic mutron op or ura a hv oken tu mar. rffoctivo tn producing 10- apecitior thn chronic X-ray azposurar, f.a., thoro fur born 1088 racovory aftor each putid doso of nut r wu. ducing offrets appou roughly caapu.blo. Tho w o r 4 1 turror incldence, hoY.vor, I 8 too low to -0 poritivr rtrtuwntr. h thal it y and twt proRrpottr In tho 1Ftoratur0, baed upon 1fPit.d mamkr o f a aim la, irdimw tht th. gomral foaturar o f the rym iron produced by uhok body kr dh tion vith dons in excora of 10,ooO run bo dupllcatad by irradiation o f tho hwd Jon. Tho gmn8r.l fratmoa o f tha fllneer urd rnothor roport i n tho Utoraturo tnficator tht tho aam iuturror a n bo produd by ktadhting tho rusp dorm. An rvalucrtion o f thoem conflicting rapid, wholo body (urr irradiation i n thi8 hborataty wmod a8 oxcmllont

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t M throe to four d8y death is a gut do8t.h. mu-onr h8ve a higher RBE on tna gut t h n tney do on body syrkms resroa than do X rays. Reports in the literature irrdicrk tht Thorefore, it my ktht Rata ware aposod to dosor of tnermal nrutrons 8lsd X rays corrivo &yr aftor irradiation, tne intrrtLnr wore romood from pylorua to U oo co c il junctura, wanhad, and uei ghd. that th.ra MY k grater weight loa8 nftor mutram t h n aftor X rays. Thir study, kgun in 1952, y.0 co@std i n tho part yur. mn 8ttopb of th. tort ir of tho CF1 mourn waa ursd 88 th. bi of ogi al - at syr tu ~8 x lo9 thorrl n/cm2 produced an effoct oquivalrnt to d ~ p t hof tha trak8 with tospect t o the region of peak noutran collision, donrlty calcubtion of the RaE of thermal neutrons @YO Vrluos of 1.2 - 2.5 w i t h 1.3 king tha mro likely figure. Dspanjing 011 vu i our asrumptiom regarding

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bother system k i n g conaidord for an REE dotum.ilrtion Curvrr h v o boon uorkod out Dour of ktuean 100 arrd 400 r doprerr tho formtion of antibodirr i n incrwsing doper if t b acrtiyn i r h.r ken 8tldi.d extonaively i n the rat. is king dovelopad prapuatory to a coaqulron of t& of foc tr of nrutron, .j. D aUr Pint ion of ME vith tha Uw of A h Iaukri. I n CFL CF1 dco are king lnocutkd vith aa Km mwo rtnin of laukuia UT oxporure t o varying dowr of X rayr a d nutronr In aa a t Wt to dotormino MRE vith the por cent loukd ~-8 u an o d point. A prelklruy r t d 7 with X rays irdicatar 100 por cont *or follovirq I00 r Dabout 90 par coat toUou1ng 300 r, a b u t 60 p r cont Pollaring 200 r, and about 30 p r cont folloulng 100 r. There i r mrml trlto in control CPl mica of about 20 per cont. Pnparationr are urd.? m y for a cornbind X my and nutron rtudy. ioniring radiations wing uptrko of +-nicotinic acid Sovd atkmpte uero do tod rta ~ i ntha h& of WioW

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avry tochniul difficultior vith ea~posuretechniqws were oncountereb. Difficulty i n reputing the gamum ray dclras u it hal rt in q Co@ 8ad Ra sourcaa h r boon tho grwtrrt problom. Scan attrmptr to uro tritium a d dotoralno tha Rffl of tritium beta particlor have hndo, but the work i r tamporuilt in .bpneo until the cohlt wurco …