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MONTHLY STATUS AND PFtOGRESS REPORT Top Secret Appendix Under Separate Cover Budget and Reports Division
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loci in mice produced additgonal evidence that the mutation rate at this dose i s lower than expected, on the hypothesis of linearity, from the 60C-r rate previously determined. The rates for doses lolver than 600 rmy be higher than expected from the 600-r rate - Preliminary data from a 300-r experiment shsw a total of 14 presumed mutations9 a t the seven loci tested, in a total of 23,025 offspring exanined- Tn is rate is slightly,but not significantly, higher thar, eq ec ted on the hypothesis of linear increase between 0 r and 600 r. Final conclusions on the shape of the curve must await the testing of the mutants and the col-lectj.on of more The ? G O - r d a t a support the most important
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conclusion regched from the 6@-r data, namely, that the i maced mutation rate i n t h e mouse 1s approximately.one order of magnitude higher than the rates obtained from similar ex per bents on Drosophila, iiadiation Pro tection-=Studies on the release of material (absorbing in then lt rav fo let) from X-irradiated Escherichia -.c oli, have shown that the phosphate concentration of the suspending medium has an important influence 02 this release of material, presence of relatively high concentrations, this release is a hOSt completely A relationshi2 between phosphorylation and the development of ttleakagett i n XIrradiated cells is suggested by these data, In the absence cf added phosphate orin the Arsena%e i n proper concentration antagonizes the release of material. Purification of a factor from beef spleen wnich effects bacterial recovery from irradiation has been attempted by using a 90 percent ethanol extraction of the hot water extract from homogenized decapsulated spleen, A pool of this filtrate is being accumulated and dried by ly oph i liz ation for use a s source material for further purification by several methods inck ding solvent extraction, charcoal adsorption, and chromatography, Preliminaqr experiments with adsorption on charcoal and el uti on with absolute ethanol indicate that another several-fold purification may b e accomplished in a single step, px if i ed material is destroyed by ionizing radiation, Experiments are i n progress t o determine ifthe partially Biochemistq-The factor from hog kidney cortex responsible for the great stimclation of luminescence i n luminous bacterial extracts was identified as pal hit ic aldehyde e The molecular weight of the 2 p,!t-dinitrophenylhydrazone of kiclney cortex factor was determined by its extinction per unit weight, and its identity was checked by mixed melting points with a synthetic sample of the pah it ic aldehyde derivative, Biophysics--Czlibration of the f a s t neutron counting equipment by the associated D article method using the D-D reaction with the new Cockroft3alton accelera'tor i s about 70 percent complete butZon have been ob take d and extrapolated values yield counting efficiencies for the counter within about 8 percent of the published values for hs tru - mefits of t h e same type, P o 4 e rieutror, source has been obtained, Good agreement with the output of a standardized Xath for Bio fc --Data, obtained from mice exposed in a recent weapons tes q-+i n evada and fro= mice exposed t o neutrons inthe ORNL 86inch cyclctron, Viere compared t G provide neutron dose estimates. factory least squares fits were computed for both sets of data and from these, dose es timtes' were derived e
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The following problem has been solved (by Iiumerica> integration) the total counts, registered by an immersion counter, which is derived from radioactive material located at any given distance from the counter. results were computed for! - particles in bone and compared with those from an approximate formulation available i n the literature. It i s required t o compute that fraction of Use of Radioisotopes i n Uedicine--The Oak Ridge Institute of Nuclear Studies conducted a course i n the techniques for using radioisotopes i n medicine September 14-25, Enrollment i n the course totaled 122.