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ATOMIC ENERGY COMMISSION The claims in the subject patent were proofread and the fol lo cinq Claim 1, column 6, line 70, ---of said result in a species--- should have been inserted after "separation." This insertion was requested by Examiner's he nd ment dated 140 Certificate of Correction was deemed necessary by the attorney,
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We have been advised by the Assistant General Counsel for Patents, Headquarters, that PATENT NO. 3 3 1 3,400 , issued July 7, 1970, on your invention report C N I D 1613 & SUPPI .
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ATOMIC ENERGY COMMISSION David S. Zachry, olief Oak Ridge Patent Group There i s enclosed herewith for your files a copy of the patent which recently Issued on the above applfcation. It would be apprec1at.d if you w u l d forward the extra copy of the cnclosed patent to the inventor ciation for the reporting of the subject invention. and express our appreAssistant General Counsel
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ATOMIC ENERGY COMMISSION Oak Ridqe Patent *oup Tranmi tted herewith for your infonation an& t o complete your files for the above application are copies of the papers sent t o us by the Exaniner Kith the informal note dated April 13, 1970. I t is regretted that we had failed t o furnish these copies heretofore. Assistant General Counsel
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I n response to your memorandum o f March 19, 1970, this application has been reviewed and no amendment under 312 I s deemed necessary. An abstract o f the application i s enclosed. Oak Ridge Patent Group
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TI t l a :. A METHOD OF CEHTRIFUGAL SEPARATION AND RECOVERY OF CHEMICAL SPECIES UTILIZING A LIQUID MEDIUM A method for sep arat lng chemical specles from a mixture by can trl fuga tl on of the mlxture through all qu ld dens l ty grad lent contalnlng chenical reagents arranged I n zones w l th fn the density gradient to provlde a pre-datermfned sequence of ch d cal tmattmts 1s dercrlbed. The method Is based upon the prlnciple that nacropartlculate materlal Is caused t o sediment Ina centrifugal field, whlle molecular rlte mater1al I n solutlon Is relatlvcly unaffected. Thus , chemically active reagents I mbi - lltad in aden sl ty grad tent can be arranged to provfde a prcselected sequence o f steps in as e par atl on process involvtng partlalate matter. Apart l cula rly useful app li cat fon of the inventlon is I n the sep arat lon of mlxed callolds. For examde, proteln mixtures can be preclpltated I n an upper zone, and separated by red fm nt atl on rate dffferences, by lsopycnlc bandfng, o r by
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ATOMIC ENERGY COMMISSION Oak Ridge Patent Group Where was received in this office one copy of the Notice of clalmr hve been allow d In the rub j ect application. It l r requerted that you review the application and determine whether or not an amendment under Rule 312 i r deaped necessary. Unlerr an amendment is received in 30 d8ys frm the date of the Notice of Allowance, the application will pars t o issue. Assistant General Counsel
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ATOMIC ENERGY COMMISSION David S. Zachry, Chief Oak Ridge Patent Group There is enclosed he-with for your files a copy of the Preliminary Notice of Allowance for the subject Assistant General Counsel As noted above
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R. A . Anderson, Assistant General Counsel APPL. SER. rjo. A METHOD OF CENTRIFUGAL SEPARATION AND RECOVERY OF CH03IW SPECIES UTILIZING A LIQUID MEDIUM amendment i n the above docket. The amendment: aims i n excess o f those previously paid for. Response is due in the Patent Office. by December 22, 1969- ) Since this application was filed prior t o the effective eate of the new fee bill, no fees are required with this amendrent. Ridge Patent Grouo
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ATOMIC ENERGY COMMISSION Oak Ridge Patent Group FOR: METHOD OF CENTRIFUGAL SEPARATION AND RECOVERY OF CHEMICAL SPECIES UTILIZING A LIQUID MEDIUM There is enclosed herewith for your study and consideration a copy of an Office Action inthe above-identified application dated September 22, 1969, together with t h e necessary references cited there in. Please note that due toa shortened time for reply,the amendment will be due in the U. S. Patent Office by December 22, 1969. Assistant General Counsel Patent Office Action
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APPL. SER. YC.: 612,207 F@P: A YETHM OF CENTPIFUGAL SEPARATION AND RECOVERY OF CHEPICAL SPECIES UTILIZING A LIqUID MEDIUM Enclosed are an amendment and a Draftsman's anendnent in the above docket. The amendment adds no clalms i n excess o f those previously Ye have enclosed only one copy o f pertinent portlons of the "National Cancer Institute Monograph 21" rPquerted by the Examiner, because of the large number o f pages Involved. Ye have a copy of the monograph I n our files I n the event that you desire a copy for your records. Response I s due I n the Patent Office by April 16, 1969.
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ATOMIC ENERGY COMMISSION Oak Ridge Patent Group For: METHOD OF CENTRIFUGAL SEPARATION 61 RECOVERY OF CHEMICAL SPECIES There i e enclosed herewith for your study and consideration a copy of an Office Action inthe above-identified application dated January 16, 1969, together with the necessary references cited therein. Please note that due toa ehortened time for reply, the amendment vi11 be due i n the U. S. Patent Office by April 16. 1969. A so iet mt General Couneel Patent Office Action
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A patent application was filed ,. in the U. S. Patent Office on the above invention, under the t i t l e "A MstM of and aasigned Serial Number 61Chief, Oak Ridge Patent Group
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Oak Ridge Patent Group General Counsel for Patents The application on t k above-Identifled docket was tiled In the United Sts tet3 %tent Office January 25, 1967, and assigned Serial No. 612,297. The patent Office baa Initlally aat3lgned t h i s case to 'Ihir case does not require a Secrecy Order. Buy U.S. Savings B0nd.r Rcgnlariy on the Payroll Sauings Plan
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UNITED STATES GOVERNMENT for Patents, Germantown PROM : Do S o Zachry, Chief, O a k Ridge Patent Group Enclosed are an application in the above case and the following ( Record of Invention Fees payable arc: Total claims in excess of ten IC.00t imer $ 2 * 00....0.0...0.0 Number of independent claims minus one times $10.00 ..,........ 67. OOT o tal Filing Fee,,.,...... $ Filing prior to is nece8su-y. As stated above
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UNION CARBIDE CORPORATION ” Oak Ridge, Tennessee 37830 Oak Ridge Patent Group Reference is made t o Mr. Zachry’s letter of January 11, 1967, transmitting a patent application and formal papers inthe These documents have been executed and a r e enclosed. The Assignment has been approved and consented to by I)r. C. E. Larson on behalf of Union Carbide. Ycurs very truly,, Legal and Information Con tr ol Department
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Union Carbide Corporation Post Office Box P Oak Ridge, Tennessee 37830 Enclosed a r e a patent application and fonnal papers for execution by t h e appropriate Carbide employees. Please return the executed material a t your earllest opportunity so t h a t we may secure the benefits of an early filing date i n the U. S o Patent Office. Very truly yours, Oak Ridge Patent Group Oak Ridge Operations
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UNION CARBIDE CORPORATION Oak Ridge, Tennessee 37830 Oak Ridge Patent Group The second draft of a proposed application for patent in the referenced case has been reviewed by our Mr. Cline in consultation with appropriate Changes pertaining to page 3, paragraph 3are suggested inan enclosure. The description of Fig. 4 which appeared on page 8has been changed t o conform to a simplified representation (enclosed). included i n an amended and expanded version of Example I V which is also enThis description has been It is suggested that in preparing Fig. 4in final form the drawing be suffic i ent ly enlarged in order to show the necessary detail within the rotor should ‘ also the feed liquid within the conduit 3. However, the feed liquid in the region indicated by 10 should be stipple shaded t o pure white i n the upper portion, indicating complete transfer of particles into the gradient. The bands 12 in the gradient9 should be evenly stripped, a s - If there are questions concerning the revision of the second draft of the proposed patent application,or if additional assistance is desired i n placing the application in final form, please call upon Mr. Cline. Yours very truly, Legal and Information Page 3, paragraph 3, revised Example IV, revised
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UNION CARBIDE CORPORATION If them are qwrtiomr cooc8rni.ag the rnviriaa of tho w c d drrrft of tb.
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I n the drawings, Fig. 1Ais an elevation of a centrifuge vessel with a density gradient liquid overlayered with a less dense sample. Fig. 1 B sharrs the vessel and contents of Fig. IA with the sample diffused in the gradient. Fig. 1C is the vessel and contents of Fig. LA after centrifuging. Fig. 2 A is an elevation of a centrifuge vessel containing a non-aqueous liquid of radially increasing density overlayered with an aqueous sample and a nonaqueous precipitant. Fig. 2 B shows precipitation in the aqueous layer following inter diffusion of the overlayers. Fig. 2 C shows the vessel and contents with isopycnic separation of precipitated solutes following cent rif u - gation. Fig. 3A i s an elevation of a centrifuge vessel with a non-aqueous gradient and a n aqueous overlayer containing a multiplicity of solutes. Fig. 3B i s the vessel and contents of Fig. 3A after initial centrifuging and inter diffusion of the aqueous and non-aqueous layers. Fig. 3C is the vessel and contents of Fig. 3B showing isopycnic banding of precipitated species after final centrifuging. Fig. 4 is a schematic of a bawl-type,continuousflow centrifuge system for carrying out my process.
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/ Util iz in s cent ri fuga tion the system of Anderson, etal, wherein continuous flow i s combined with isopycnic banding of sedimented part icu - Monograph 21, pages 199-216), a suspension of calf serum proteins was aggregated, sedimented and recovered in three distinct density zones as reso lub ili zed protein fractions. core, which is not apart of this invention, is adapted t o provide for Referring t o Fig. 4 a B-IX type high-volume, continuous flow-through of feed liquid centripetal to an i m - prisoned density gradient of low volume. The rotor comprises a hollow *bowl 1, a tapered core 2, a first axial conduit 3with radial branches 4, communicating through a face seal5with outside feed supplying means (not shown) and with a liquid containment cavity6inside the rotor, and a second axial conduit 7with radial branches 8concentric with the first conduit and cmu nica ting also through said face seal5 with outside liquid receiving means and with said liquid containment cavity 6. An aqueous sucrose gradient9increasing in density radially was pumped into the core and established in the centrifugal portion of the liquid containment cavity a tarot or speed of less than SO00 ppm. A suspension of calf serum prote in s in phosphate buffered saline solution, which was first caused t o aggregate by making the medium 70 per cent with respect to ethanol, was pumped through the rotor at a rate of 3liters per hour a tarot or speed of 40,000 centripetal portion of the liquid containment cavity 10 and exiting through the second axial conduit 7. The aggregated protein particles were thus sedi - mented into the imprisoned aqueous gradient. When the gradient was recovered (by centripetal displacement witha high density liquid) an optical density tracing of the effluent gradients h wed different chemical species at three iso py c nic level3 This indicated that the three different chemical specie8 were first banded ‘ iso py c nic ally as particles ad later reso lub ili zed in the
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HCP:Cd Oak Ridge, Tennessee Union Carbide Ccrporation Post Offire Box P Oak Ridge, Tennessee This office has prepared and endooees a second draft of a propcsed application f o r patent in the above case. It will be appreciated if the inventor, In revfewing thir d r a f t , will give special attention t o the accuracy of the brief de script icc o of the newly substituted figures of the drawings located on page 3 of the specification. klhen the inventor has campleted his review of the retfised drm, t t will be appreciated if you will return the enclosed material alorg with the invantor's cements. corrections uiay be made direct iy in the draft. mor changes, additions, or oak Ridge Patent Group Oak Ridge Operations
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UNION CARBIDE CORPORATION Oak Ridge, Tennessee 37833 Oak Ridge Patent Group Reference i s made t o Mr. Zachry's letter of Nov. 2, 1966, trans - mitt inga preliminary draft of a patent application relating to the The unusual nature of the technology involved i s recognized asa difficulty in preparing a patent application of broad scope. This stems from the facts that the concept i s fundamentally new, the applications are far reaching, but the inventor ' s investigation is cm par at iv ely limited. in ary draft and have made suggested revisions in separately typed notes included herewith. The specification and claims have been reworked t o broaden the scope of the invention t o include the concepts disclosed inC NID 1969 while more closely integrating subject matter thereof with that of CNID 1613 and recent work performed i n Dr. supplied as illustrating several workably known variations. added examples were recently performed and are in substitution of those examples of a preliminary nature included in the disclosures relating to this application. The figures and appertaining descriptions are If there are questions concerning the proposed revision of the patent application, or if additional assistance is desired in placing the application in final form, please call upon Mr. Cline. Legal and Information
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YAor changee, additions, o r dcleticns c81: tede directly 3n the Major alterations or suggesticris snculd be by uzq of a
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It will be greatly appreciated if you id-1return the enclosed opportunity t o review the enclosures. desirable in this case, a representative iron: th h office Kill be availrrble for tlfit, purpose. Very truly yours, Oak Ridge Pateqt Group
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Oak iidze Patent ;roup 3ctcSer 26, 1955 Assistant Scneral Counsel f o r Patents These cases have been reviewed and we are disposed t o agree with your recommendation t o close S-33#395 and combine the subject matter therein S-33,395 will therefore be closed and filed with those cases not to Se
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uah Xiige Fatact Group X fur%her renew of :-,-3$j5> by t h e attorncy przpnrimg the authcrl ed apFl;caticr. in :-:7,0>/. lnuieates that one ad the Ewe in-rention is nunbered, last raceAvSd docAet should be included in the ear her ab58 ard Lhat the subject matter 02 the kigherAccordi:gly, we Dlar!to file a singla application disclosing the sub2xt
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